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KMID : 1134819980270030441
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Journal of the Korean Society of Food Science and Nutrition
1998 Volume.27 No. 3 p.441 ~ p.447
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Isolation and Cultivation of Microorganism Producing Levanbiose from Levan
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Kang Eun-Jung
Kang Soo-Kyung
Lee Tae-Ho
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Abstract
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A bacterial strain No. 43 was isolated from soil samples as a levan-assimilating microorganism producing an extracellular levanase and hydrolying levan to levanbiose. According to the taxonomic characteristics of its morphological and physiological properties, the strain was identified as Pseudomonas sp. No. 43. The optimum cultural medium was composed of 10g levan, 5g(NH₄)SO₄, 3g NH₄Cl, 3g polypepton, 1g K₂HPO₄, 0.5g MgSO₄. 7H₂O, and 0.2g MnCl₂ . 4H₂O per liter. The cultivation for levanase was carried out in pH 7.0 at 40℃ for 28 hr. The reaction product was a kind of oligosaccharide and it was purified by chilled ethanol precipitation and gel filtration for evaluation of degree of polymerization (DP). The purified product was determined as levanbiose of MW 342 and DP2 by HPLC and FAB-MS.
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KEYWORD
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F2, levanase, levanbiose, levanoligosaccharide
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